How many letters comprised the Latin alphabet?

The Latin alphabet, which forms the basis for many modern languages including English, originally comprised 23 letters. This set included the letters A to Z, with the exception of J, U, and W, which were later additions. The letters I and V were used interchangeably in certain contexts, while J and U were separate letters only in more recent times. Hence, the correct answer reflects the historical context of the Latin alphabet before the expansions and variations that occurred in subsequent languages.